Figure 1. RNA isolation and subsequent application with RT-PCR.
(a) RNA (1 µg) isolated from P. elisabethae. 1 and 6: 1 kb DNA Plus ladder, 2-3: Frozen P. elisabethae isolated using Trizol, 4-5: Live P. elisabethae isolated using Trizol. 7: Live P. elisabethae isolated using RNeasy.
(b). Region of a differential display RT-PCR gel showing cDNA transcripts induced for terpene production in P. elisabethae and its algal symbiont. Original RNA was isolated using RNeasy method. Lane 1 P. elisabethae (control), Lane 2 Purified Symbiodinium sp. (control), Lane 3 and 4 P. elisabethae cDNA transcripts representing differential gene expression responses from the addition of 150 µM Methyl Jasmonate.