Table 1. Summary of results from RNA extractions. Total time = includes Percoll cell isolation (if required), RNA isolation, DNase treatment if needed, and analysis by spectrophotometry and gel electrophoresis. n = 4, * n = 3, ^# n = 2

Method

Sample

RNA yield
µg mg^-1 tissue or
individual cell

A₂₆₀:A₂₈₀
ratio

Bench time (hr)

Total time (hr)

RNeasy

P. elisabethae frozen

0.055

1.39

1 hr

3 hrs

Trizol

P. elisabethae frozen

1.039

2.33

6 hrs

9 hrs

RNeasy

P. elisabethae live

0.228

2.15

1 hrs

3 hrs

Trizol

P. elisabethae live

0.536

2.46

6 hrs

9 hrs

RNeasy

Symbiodinium frozen

2.693 x 10^-7

1.55

1 hr

8 hrs

Trizol

Symbiodinium frozen^#

1.178 x 10^-6

2.42

6 hrs

14 hrs

RNeasy

Symbiodinium live

2.556 x 10^-7

1.30

1 hr

8 hrs

Trizol

Symbiodinium live*

7.880 x 10^-7

2.28

6 hrs

14 hrs