Table 3. 23 Complete factorial design experiment for YE, MWR and FBS supplements. Cells were inoculated in the different media at a final concentration of 2.5 x 105 cells.ml-1 and cultivated at 28º C.


Assay

Independent Variables

Response Variables

 

YEa  (g.l-1)

MWRb  (%w/v)

FBSc  (%v/v)

Xsd (x105 viable cells.ml-1)

Xmee (x105 viable cells.ml-1)

Vmef (%)

µg (h-1)

1B

4

3

1

35.2

12.9

91

0.0147

2B

8

3

1

34.2

10.1

88

0.0141

3B

4

5

1

37.5

10.1

91

0.0198

4B

8

5

1

0.0

0.0

0

0.0000

5B

4

3

3

31.1

13.4

92

0.0216

6B

8

3

3

25.0

8.5

78

0.0182

7B

4

5

3

27.5

9.8

85

0.0187

8B

8

5

3

20.1

9.7

82

0.0150

9B

2.6

4

2

37.5

19.5

92

0.0141

10B

9.4

4

2

14.0

5.4

62

0.0154

11B

6

2.3

2

54.6

13.2

94

0.0154

12B

6

5.7

2

22.4

8.7

82

0.0138

13B

6

4

0.3

33.1

7.9

90

0.0138

14B

6

4

3.4

34.2

13.8

93

0.0138

15B

6

4

2

29.2

9.8

86

0.0169

16B

6

4

2

20.1

8.0

84

0.0141

17B

6

4

2

25.0

9.5

88

0.0133

Grace's + FBS

-

-

10

14.7

8.1

96

0.0231

Sf900II

-

-

-

41.0

14.6

66

0.0198

Data presents the ranges of the independent variables ayeast extract; bmilk whey retentate and cfetal bovine serum used to optimize the dependent variables dviable cell concentration in stationary phase, eviable cell concentration in midexponential phas, fviability in midexponential phase and gspecific cell growth rate.


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