Table 3. 23 Complete factorial design experiment for YE, MWR and FBS supplements. Cells were inoculated in the different media at a final concentration of 2.5 x 105 cells.ml-1 and cultivated at 28º C. |
Assay |
Independent Variables |
Response Variables |
|||||
|
YEa (g.l-1) |
MWRb (%w/v) |
FBSc (%v/v) |
Xsd (x105 viable cells.ml-1) |
Xmee (x105 viable cells.ml-1) |
Vmef (%) |
µg (h-1) |
1B |
4 |
3 |
1 |
35.2 |
12.9 |
91 |
0.0147 |
2B |
8 |
3 |
1 |
34.2 |
10.1 |
88 |
0.0141 |
3B |
4 |
5 |
1 |
37.5 |
10.1 |
91 |
0.0198 |
4B |
8 |
5 |
1 |
0.0 |
0.0 |
0 |
0.0000 |
5B |
4 |
3 |
3 |
31.1 |
13.4 |
92 |
0.0216 |
6B |
8 |
3 |
3 |
25.0 |
8.5 |
78 |
0.0182 |
7B |
4 |
5 |
3 |
27.5 |
9.8 |
85 |
0.0187 |
8B |
8 |
5 |
3 |
20.1 |
9.7 |
82 |
0.0150 |
9B |
2.6 |
4 |
2 |
37.5 |
19.5 |
92 |
0.0141 |
10B |
9.4 |
4 |
2 |
14.0 |
5.4 |
62 |
0.0154 |
11B |
6 |
2.3 |
2 |
54.6 |
13.2 |
94 |
0.0154 |
12B |
6 |
5.7 |
2 |
22.4 |
8.7 |
82 |
0.0138 |
13B |
6 |
4 |
0.3 |
33.1 |
7.9 |
90 |
0.0138 |
14B |
6 |
4 |
3.4 |
34.2 |
13.8 |
93 |
0.0138 |
15B |
6 |
4 |
2 |
29.2 |
9.8 |
86 |
0.0169 |
16B |
6 |
4 |
2 |
20.1 |
8.0 |
84 |
0.0141 |
17B |
6 |
4 |
2 |
25.0 |
9.5 |
88 |
0.0133 |
Grace's + FBS |
- |
- |
10 |
14.7 |
8.1 |
96 |
0.0231 |
Sf900II |
- |
- |
- |
41.0 |
14.6 |
66 |
0.0198 |
Data presents the ranges of the independent variables ayeast extract; bmilk whey retentate and cfetal bovine serum used to optimize the dependent variables dviable cell concentration in stationary phase, eviable cell concentration in midexponential phas, fviability in midexponential phase and gspecific cell growth rate. |
|