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Table 4. Kinetic and economic parameters for Sf9 cells in selected media after cell adaptation. Cells were inoculated in media 4A, 16A, 9B and 11B at a concentration of 3 x 105 cells.ml-1 and cultivated at 28ºC and 100 rpm. |
|
Parameter |
Formulation 4A |
Formulation 16A |
Formulation 9B |
Formulation 11B |
Grace's + 10% FBS |
Sf900II control |
|
Viable cell concentration at midexponential phase (105 cell.ml-1) |
13.8 |
14.0 |
6.3 |
5.8 |
5.8 |
18.0 |
|
Viable cell concentration at stationary phase (105 cell.ml-1) |
37.5 |
34.2 |
23.0 |
27.5 |
9.5 |
80.0 |
|
Specific cell growth rate (h-1) |
0.014 |
0.020 |
0.016 |
0.021 |
0.015 |
0.028 |
|
Population doubling time(h) |
50 |
35 |
43 |
33 |
47 |
25 |
|
Glucose specific uptake rate (10-10g/cell x h) |
4.0 |
5.6 |
3.1 |
4.6 |
2.1 |
4.5 |
|
Glutamine specific uptake rate (10-11g/cell x h) |
2.0 |
2.9 |
0.9 |
0.0 |
5.0 |
4.5 |
|
Lactate specific consumption rate (10-11g/cell x h) |
3.4 |
4.9 |
12 |
44 |
98 |
-5.7a |
|
Ammonia concentration on day 12 (mg.l-1) |
4.2 |
ND |
4.5 |
6.1 |
5.1 |
66.2 |
|
Osmolality (mOsm.kgH2O-1) |
408 |
427 |
379 |
400 |
326 |
350 |
|
Media cost (US$ per liter) |
38.30 |
38.80 |
34.80 |
30.20 |
23.00 |
44.00 |
a Negative sign indicates production. |
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