|
| Table 5. Polyhedra production in formulations 4A, 11B, Grace's medium supplemented with 10% FBS and Sf900II medium. Cells were inoculated at a concentration of 3 x 105 cells.ml-1 and cultivated at 28ºC and 100 rpm. Sf9 cells at a final concentration of 1.0 x 106 cells.ml-1 were infected with baculovirus during the midexponential phase, at a MOI of 1, and further cultivated at 28ºC. |
|
Parameter |
Medium formulation |
|||
|
4A |
11B |
Grace's + 10% FBS |
Sf900II |
|
|
Baculovirus produced (106 polyhedra.ml-1) |
16.0 |
6.6 |
6.1 |
18.0 |
|
Cost for 1010 polyhedra (US$) |
23.94 |
45.76 |
37.70 |
24.44 |
|
|