Figure 1. Native strain and C. acetobutylicum ATCC 824 DNA restriction fragments digested with Eco52I separated by PFGE.
(a) Lane 1: Lambda–HindIII marker; 2: Lambda concatamers (NEB); 3: C. acetobutylicum ATCC; 4: IBUN 22A; 5: IBUN 125C; 6: IBUN 140B; 7: Lambda concatamers (NEB);  8: IBUN 62F; 9: IBUN 95B; 10: IBUN 13A; 11: IBUN 18A; 12: Lambda–HindIII marker; 13: IBUN 18S; 14: IBUN 62B; 15: IBUN 158B; 16: IBUN 18Q; 17: Lambda–HindIII marker, 18: IBUN 64A; 19: IBUN 137K; 20: IBUN 137K Pulse time of 1 to 8 sec for 16 hrs.
(b) Lane 1: Lambda-HindIII marker; 2: C. acetobutylicum ATCC; 3: IBUN 22A; 4: IBUN 125C; 5: IBUN 140B; 6: IBUN 62F; 7: IBUN 95B; 8: IBUN 13A; 9: Lambda concatamers (NEB); 10: IBUN 18A; 11: IBUN 18S; 12: IBUN 62B; 13: IBUN 137K; 14: IBUN 158B; 15: IBUN 18Q; 16: IBUN 64A; 17: Lambda HindIII marker.Pulse times of 1 to 40 sec for 22 hrs.
(c) Lane 1: IBUN 125C; 2: C. acetobutylicum ATCC 824; 3: IBUN 22A; 4: IBUN 140B; 5: IBUN 62F; 6: IBUN 13A; 7: IBUN 18A; 8: Lambda concatamers (NEB); 9: IBUN 18S; 10: IBUN 62B; 11: IBUN 137K; 12: IBUN 158B; 13: IBUN 64A; 14: IBUN 965B; 15: IBUN 18Q; 16: IBUN 125C; 17: Lambda concatamers (Promega). Pulse time of 10 to 120 sec for 22 hrs.