Figure 2. PFGE with total extracted DNA. Lane 1: C. acetobutylicum ATCC 824; 2: C. acetobutylicum ATCC 824; 3: IBUN 22A; 4: IBUN 158B; 5: IBUN 13A; 6: IBUN 18A; 7: IBUN 137K; 8: Lambda concatemers. The arrow shows: extrachromosomal DNA. Separated by PFGE, pulse times were ramped from 6 to 16 sec for 22 hrs at 200 volts.