Figure 3. pSOL1 975 bp amplified fragment. Lane 1, C. acetobutylicum ATCC 824–25 ng; 2, C. acetobutylicum ATCC 824–90 ng; 3, IBUN 18A; 4, Lambda with HindIII and EcoRI. PCR amplification for 30 cycles, denaturing at 94ºC for 1 min, aligning at 40ºC for 1 min and extension at 72ºC for 1 min using PADC-UP and PADC-DN primers.