Table 1. Number and sizes of restriction fragmentsa with C. acetobutylicum ATCC 824 genomic DNA. |
Fragment |
ApaI |
ApaI |
Eco52I |
Eco52I |
SmaI |
XhoI |
1 |
706 ± 21 |
x |
681 |
1,355 |
713 |
692 |
2 |
657 ± 25 |
x |
592 |
530 |
654 |
620 |
3 |
523 ± 22 |
523 ± 22 |
465 |
485 |
628 |
506 |
4 |
485 ± 12 |
485 ± 12 |
201 |
440 |
484 |
454 |
5 |
413 ± 18 |
431 ± 18 |
184 |
390 |
316 |
404 |
6 |
379 ± 19 |
379 ± 16 |
149 |
275 |
274 |
352 |
7 |
352 ± 15 |
352 ± 15 |
106 |
270 |
259 |
322 |
8 |
295 ± 22 |
295 ± 18 |
74 |
200 |
215 |
265 |
9 |
283 ± 16 |
283 ± 16 |
13 |
145 |
187 |
229 |
10 |
212 ± 11 |
212 ± 11c |
10 |
55 |
150 |
170 |
11 |
192 ± 12 |
192 ± 12 |
|
|
102 |
125 |
12 |
181 ± 12 |
181 ± 12 |
|
|
71 |
109 |
13 |
136 ± 12 |
136 ± 12 |
|
|
56 |
73 |
14 |
109 ± 9 |
109 ± 9 |
|
|
|
13 |
15 |
88 ± 11 |
88 ± 11 |
|
|
|
10 |
16 |
71 ± 7 |
71 ± 7 |
|
|
|
|
Estimated |
5,081 |
3,931 |
2,455 |
4,145 |
4,115 |
4,351 |
astandard deviations were obtained from three determinations. Fragment sizes were determined with Lambda molecular markers (NEB). baccording to Wilkinson and Young, 1993. cdouble band. daccording to Cornillot et al. 1997b. |
|