Figure 3. Comparison of methods to evaluate the viability.
(a) In protoplasts.
(b) Cell suspensions. Methods of viability estimation: 1. Evans Blue. 2. MTT (room temperature). 3. MTT 15 min at 37ºC. 4. MTT 30 min at 37ºC. 5. MTT 120 min at 37ºC.