Table 1. Coded levels and real values (in parentheses) for the first factorial design (17 trials) and glucosyltransferase activity (U/mL).


Assay

Variable (g/L)

Enzyme activity

(U/mL)

SCMa

BPb

YEPc

1

-1 (130.36)

-1 (18.10)

-1 (37.14)

5.63

2

+1 (219.64)

-1 (18.10)

-1 (37.14)

0.13

3

-1 (130.36)

+1(41.90)

-1 (37.14)

5.97

4

+1 (219.64)

+1(41.90)

-1 (37.14)

0.20

5

-1 (130.36)

-1 (18.10)

+1 (72.86)

0.63

6

+1 (219.64)

-1 (18.10)

+1 (72.86)

0.28

7

-1 (130.36)

+1(41.90)

+1 (72.86)

0.32

8

+1 (219.64)

+1(41.90)

+1 (72.86)

2.12

9

-1.68 (100)

0 (30)

0 (55)

2.26

10

+1.68 (250)

0 (30)

0 (55)

0.38

11

0 (175)

-1.68 (10)

0 (55)

0.41

12

0 (175)

+1.68 (50)

0 (55)

1,19

13

0 (175)

0 (30)

-1.68 (25)

0.85

14

0 (175)

0 (30)

+1.68 (85)

0.40

15

0 (175)

0 (30)

0 (55)

0.42

16

0 (175)

0 (30)

0 (55)

0.47

17

0 (175)

0 (30)

0 (55)

0.48

asugar cane molasses; bbacteriological peptone; cyeast extract Prodex Lac SD®.

Supported by UNESCO / MIRCEN network