Table 1. Coded levels and real values (in parentheses) for the first factorial design (17 trials) and glucosyltransferase activity (U/mL). |
Assay |
Variable (g/L) |
Enzyme activity (U/mL) |
||
SCMa |
BPb |
YEPc |
||
1 |
-1 (130.36) |
-1 (18.10) |
-1 (37.14) |
5.63 |
2 |
+1 (219.64) |
-1 (18.10) |
-1 (37.14) |
0.13 |
3 |
-1 (130.36) |
+1(41.90) |
-1 (37.14) |
5.97 |
4 |
+1 (219.64) |
+1(41.90) |
-1 (37.14) |
0.20 |
5 |
-1 (130.36) |
-1 (18.10) |
+1 (72.86) |
0.63 |
6 |
+1 (219.64) |
-1 (18.10) |
+1 (72.86) |
0.28 |
7 |
-1 (130.36) |
+1(41.90) |
+1 (72.86) |
0.32 |
8 |
+1 (219.64) |
+1(41.90) |
+1 (72.86) |
2.12 |
9 |
-1.68 (100) |
0 (30) |
0 (55) |
2.26 |
10 |
+1.68 (250) |
0 (30) |
0 (55) |
0.38 |
11 |
0 (175) |
-1.68 (10) |
0 (55) |
0.41 |
12 |
0 (175) |
+1.68 (50) |
0 (55) |
1,19 |
13 |
0 (175) |
0 (30) |
-1.68 (25) |
0.85 |
14 |
0 (175) |
0 (30) |
+1.68 (85) |
0.40 |
15 |
0 (175) |
0 (30) |
0 (55) |
0.42 |
16 |
0 (175) |
0 (30) |
0 (55) |
0.47 |
17 |
0 (175) |
0 (30) |
0 (55) |
0.48 |
asugar cane molasses; bbacteriological peptone; cyeast extract Prodex |
|