Table 2. Coded levels and real values (in parentheses) for the second factorial design (17 trials) and glucosyltransferase activity (U/mL). |
Assay |
Variable (g/L) |
Enzyme activity (U/mL) |
||
SCMa |
BPb |
YEPc |
||
1 |
-1 (120.24) |
-1 (11.07) |
-1 (18.10) |
6.34 |
2 |
+1 (179.76) |
-1 (11.07) |
-1 (18.10) |
8.28 |
3 |
-1 (120.24) |
+1(28.93) |
-1 (18.10) |
7.56 |
4 |
+1 (179.76) |
+1 (28.93) |
-1 (18.10) |
8.97 |
5 |
-1 (120.24) |
-1 (11.07) |
+1 (41.90) |
7.39 |
6 |
+1 (179.76) |
-1 (11.07) |
+1 (41.90) |
8.68 |
7 |
-1 (120.24) |
+1(28.93) |
+1 (41.90) |
6.63 |
8 |
+1 (179.76) |
+1 (28.93) |
+1 (41.90) |
6.28 |
9 |
-1.68 (100) |
0 (20) |
0 (30) |
4.99 |
10 |
+1.68 (200) |
0 (20) |
0 (30) |
7.01 |
11 |
0 (150) |
-1.68 (5) |
0 (30) |
7.06 |
12 |
0 (150) |
+1.68 (35) |
0 (30) |
7.47 |
13 |
0 (150) |
0 (20) |
-1.68 (10) |
8.47 |
14 |
0 (150) |
0 (20) |
+1.68 (50) |
7.06 |
15 |
0 (150) |
0 (20) |
0 (30) |
8.43 |
16 |
0 (150) |
0 (20) |
0 (30) |
8.23 |
17 |
0 (150) |
0 (20) |
0 (30) |
7.83 |
asugar cane molasses; bbacteriological peptone; cyeast extract Prodex |
|