Table 3. Coded levels and real values (in parentheses) for the third factorial design (17 trials) and glucosyltransferase activity (U/mL). |
Assay |
Variable (g/L) |
Enzyme activity (U/mL) |
||
SCMa |
BPb |
YEPc |
||
1 |
-1 (130.24) |
-1 (11.07) |
-1 (9.05) |
4.23 |
2 |
+1 (189.76) |
-1 (11.07) |
-1 (9.05) |
3.97 |
3 |
-1 (130.24) |
+1(28.93) |
-1 (9.05) |
5.07 |
4 |
+1 (189.76) |
+1 (28.93) |
-1 (9.05) |
3.87 |
5 |
-1 (130.24) |
-1 (11.07) |
+1 (20.95) |
4.85 |
6 |
+1 (189.76) |
-1 (11.07) |
+1 (20.95) |
4.23 |
7 |
-1 (130.24) |
+1(28.93) |
+1 (20.95) |
5.53 |
8 |
+1 (189.76) |
+1 (28.93) |
+1 (20.95) |
3.15 |
9 |
-1.68 (110) |
0 (20) |
0 (15) |
4.89 |
10 |
+1.68 (210) |
0 (20) |
0 (15) |
3.27 |
11 |
0 (160) |
-1.68 (5) |
0 (15) |
6.14 |
12 |
0 (160) |
+1.68 (35) |
0 (15) |
4.32 |
13 |
0 (160) |
0 (20) |
-1.68 (5) |
5.19 |
14 |
0 (160) |
0 (20) |
+1.68 (25) |
4.91 |
15 |
0 (160) |
0 (20) |
0 (15) |
7.47 |
16 |
0 (160) |
0 (20) |
0 (15) |
7.12 |
17 |
0 (160) |
0 (20) |
0 (15) |
7.19 |
asugar cane molasses; bbacteriological peptone; cyeast extract Prodex |
|