Figure 4. Western blot profile comparing anti SEB antisera from different sources. Lanes 2 and 4 contain culture supernatants of SEB (~28.5kDa) producing S. aureus ATCC14458, and lanes 3 and 5 contain culture supernatants of non-enterotoxigenic S. aureus ATCC6538P. Lanes 2 and 3 were developed with anti r-SEB serum (present study); lanes 4 and 5 were developed with polyclonal antibodies (M/s Toxin Tech, USA). Lane 1 shows pre-stained molecular weight markers.