CONGRESSES, MEETINGS AND WORKSHOP PROCEEDINGS |
Centre for Biological and
Chemical Engineering Biomolecule Stabilisation From Molecular Interpretations to Bio-Industrial Applications (Calouste Gulbenkian Foundation, Lisbon, 9-12 April 2000) Programme
SUNDAY, April 9, 2000 15:00-18:00 Registration 18:00-19:00 Welcome reception
MONDAY, April 10, 2000 8:15-9:00 Registration
Opening Session 9:00-9:10 Opening remarks 9:10-10:00 Key Note Lecture – Measuring and increasing protein stability, Nick Pace (Texas A & M University, College Station, USA) 10:00-10:20 Coffee Break
Session A: Tools for monitoring protein stability Chairman: Adrie Straathof (NL) and Rui Brito (P) 10:20-10:55 Protein characterization by NMR spectroscopy, Kurt Wüthrich (ETH, Zurich, Switzerland) 10:55-11:30 Probing the stability and pH sensitivity of a triglyceride lipase, Steffen Petersen (University of Aalborg, Aalborg, Denmark) 11:30-11:55 A new method for fast determination of biocatalysts process stability, M. Boy (Graz University of Technology, Graz, Austria) 11:55-12:20 Changes in protein dynamics and stability observed by tryptophan fluorescence and phosphorescence, F. Tölgyesi (Semmelweis University of Medicine, Budapest, Hungary) 12:20-14:00 Lunch 14:00-15:30 Poster Session
Session B: Towards a molecular understanding of stabilisation Chairman: Christopher Bucke (UK) and Fernando Garcia (P) 15:30-16:05 Pressure stabilization of proteins near 100ºC: implications for structure-function and structure-stability relationships, Douglas Clark (University of California, Berkeley, USA) 16:05-16:40 Stability issues of the outer membrane protease Ompt, Maarten Egmond (University of Utrecht, Utrecht, The Netherlands) 16:40-17:00 Coffee Break 17:00-17:25 Effects of ionic species on the long-term stabilization of proteins and cells, J. de Pablo (University of Wisconsin, USA) 17:25-17:50 Ligand-induced tightening of oligomer structure: analysis of denaturation pathways reveal how oxyanions stabilise a bacterial a-glucan phosphorylase, Bernd Nidetzky (Universitat für Bodenkultur Wien, Vienna, Austria) 17:50-18:15 Thermostability engineering of fungal phytases, Roland Brugger (Hoffmann La-Roche, Basel, Switzerland) 18:15-18:40 Modules in carbohydrate-active enzymes: present views, Pedro Coutinho (IST, Lisbon, Portugal)
TUESDAY, April 11, 2000
Session C: Protein folding Chairman: Giacomo Carrea (I) and Eduardo Melo (P) 9:00-9:35 Improving the kinetic stability of an enzyme by manipulating early steps in the unfolding process, Vincent Eijsink (Agricultural University, Aas, Norway) 9:35-10:10 GimC, a novel chaperone from eukaryotes and archae that cooperates with chaperonins in protein folding, Michel Leroux (Max Planck Institute for Biochemistry, Martinsried, Germany) 10:10-10:30 Coffee Break 10:30-10:55 Quantitative assessment of complex formation of unliganded steroid hormone receptors and accompanying proteins, Alois Jungbauer (University of Agricultural Sciences, Vienna, Austria) 10:55-11:20 Protein stability in health and disease: Human transthyretin, a case study, Rui Brito (University of Coimbra, Coimbra, Portugal) 11:20-11:45 Thermodynamic stability of onconase and its mutant M23L, Giuseppe Graziano (University of Sannio, Sannio, Italy) 11:45-12:10 Folding of small membrane protein molecules, Daniel Otzen (University of Aalborg, Aalborg, Denmark) 12:10-14:00 Lunch 14:00-15:30 Poster Session
Session D: Mechanisms of stability in extreme environments Chairman: Jacques Baratti (F) and Miguel Gama (P) 15:30-16:05 Enzymes from extremophiles: the structural basis of enzyme stability and activity in extreme conditions, Michael Danson (University of Bath, Bath, UK) 16:05-16:40 Structural and genomic correlates of hyperthermostability, Christian Cambillau (AFMB-CNRS, Marseille, France) 16:40-17:00 Coffee Break 17:00-17:25 Stability and activity of mesophilic subtilisin E and its thermophilic homolog: insights from molecular dynamics, Giorgio Colombo (Istituto di Biocatalisi e Riconoscimento Moleculare, Milano, Italy) 17:25-17:50 Novel compatible solutes from hyperthermophiles: application for stabilization of enzymes, Helena Santos (ITQB, Lisbon, Portugal) 17:50-18:15 Molecular design of novel metal-binding biomolecules, oligomeric metallothioneins, Yoshikatsu Murooka (Osaka University, Osaka, Japan) 19:30-23:00 Conference Dinner
WEDNESDAY, April 12, 2000
Session E: Bio-industrial and analytical applications: case studies Chairman: Marie Dominique Legoy (NL) and Raquel Aires-Barros (P) 9:00-9:35 Stabilisation of a-amylases for starch and whole grain mash liquefaction, Sven Pedersen (Novo Nordisk, Bagsvaerd, Denmark) 9:35-10:10 Enzyme immobilisation on Eupergit C for redox biocatalysis, John Woodley (University College London, London, UK) 10:10-10:35 Stabilization of protein ligands, Martin Linhult (Royal Institute of Technology, Stockholm, Sweden) 10:35-10:55 Coffee Break 10:55-11:20 Stabilization of immobilized enzymes against organic solvents: Generation of "polymeric salts" surrounding enzyme molecules by combined use of cationic and anionic random coil polymers, José Guisan (CSIC, Madrid, Spain) 11:20-11:45 Application of solid/gas catalysis to the industrial production of esters sharing the natural label, Sylvain Lamare (University of La Rochelle, La Rochelle, France) 11:45-12:10 Transglutaminase catalyzed enzyme immobilization - an alternative approach for the development of biosensors for urea determination, Lutz Haalck (I. of Chemical and Biochemical Sensor Research, Münster, Germany) 12:10-12:30 Closing Session Contact Addresses General Information: Dr Duarte Miguel Prazeres Web Page: http://dequim.ist.utl.pt/cebq/biostabilisation |
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