Centre for Biological and Chemical Engineering
Instituto Superior Técnico, Av. Rovisco Pais
1049-001 Lisboa, Portugal
Tel.: 351-21-8419133 ; Fax.: 351-21-8419062

Biomolecule Stabilisation

From Molecular Interpretations to Bio-Industrial Applications

(Calouste Gulbenkian Foundation, Lisbon, 9-12 April 2000)



SUNDAY, April 9, 2000

15:00-18:00 Registration

18:00-19:00 Welcome reception


MONDAY, April 10, 2000

8:15-9:00 Registration


Opening Session

9:00-9:10 Opening remarks

9:10-10:00 Key Note Lecture Measuring and increasing protein stability, Nick Pace (Texas A & M University, College Station, USA)

10:00-10:20 Coffee Break


Session A: Tools for monitoring protein stability

Chairman: Adrie Straathof (NL) and Rui Brito (P)

10:20-10:55 Protein characterization by NMR spectroscopy, Kurt Wüthrich (ETH, Zurich, Switzerland)

10:55-11:30 Probing the stability and pH sensitivity of a triglyceride lipase, Steffen Petersen (University of Aalborg, Aalborg, Denmark)

11:30-11:55 A new method for fast determination of biocatalysts process stability, M. Boy (Graz University of Technology, Graz, Austria)

11:55-12:20 Changes in protein dynamics and stability observed by tryptophan fluorescence and phosphorescence, F. Tölgyesi (Semmelweis University of Medicine, Budapest, Hungary)

12:20-14:00 Lunch

14:00-15:30 Poster Session


Session B: Towards a molecular understanding of stabilisation

Chairman: Christopher Bucke (UK) and Fernando Garcia (P)

15:30-16:05 Pressure stabilization of proteins near 100C: implications for structure-function and structure-stability relationships, Douglas Clark (University of California, Berkeley, USA)

16:05-16:40 Stability issues of the outer membrane protease Ompt, Maarten Egmond (University of Utrecht, Utrecht, The Netherlands)

16:40-17:00 Coffee Break

17:00-17:25 Effects of ionic species on the long-term stabilization of proteins and cells, J. de Pablo (University of Wisconsin, USA)

17:25-17:50 Ligand-induced tightening of oligomer structure: analysis of denaturation pathways reveal how oxyanions stabilise a bacterial a-glucan phosphorylase, Bernd Nidetzky (Universitat für Bodenkultur Wien, Vienna, Austria)

17:50-18:15 Thermostability engineering of fungal phytases, Roland Brugger (Hoffmann La-Roche, Basel, Switzerland)

18:15-18:40 Modules in carbohydrate-active enzymes: present views, Pedro Coutinho (IST, Lisbon, Portugal)


TUESDAY, April 11, 2000


Session C: Protein folding

Chairman: Giacomo Carrea (I) and Eduardo Melo (P)

9:00-9:35 Improving the kinetic stability of an enzyme by manipulating early steps in the unfolding process, Vincent Eijsink (Agricultural University, Aas, Norway)

9:35-10:10 GimC, a novel chaperone from eukaryotes and archae that cooperates with chaperonins in protein folding, Michel Leroux (Max Planck Institute for Biochemistry, Martinsried, Germany)

10:10-10:30 Coffee Break

10:30-10:55 Quantitative assessment of complex formation of unliganded steroid hormone receptors and accompanying proteins, Alois Jungbauer (University of Agricultural Sciences, Vienna, Austria)

10:55-11:20 Protein stability in health and disease: Human transthyretin, a case study, Rui Brito (University of Coimbra, Coimbra, Portugal)

11:20-11:45 Thermodynamic stability of onconase and its mutant M23L, Giuseppe Graziano (University of Sannio, Sannio, Italy)

11:45-12:10 Folding of small membrane protein molecules, Daniel Otzen (University of Aalborg, Aalborg, Denmark)

12:10-14:00 Lunch

14:00-15:30 Poster Session


Session D: Mechanisms of stability in extreme environments

Chairman: Jacques Baratti (F) and Miguel Gama (P)

15:30-16:05 Enzymes from extremophiles: the structural basis of enzyme stability and activity in extreme conditions, Michael Danson (University of Bath, Bath, UK)

16:05-16:40 Structural and genomic correlates of hyperthermostability, Christian Cambillau (AFMB-CNRS, Marseille, France)

16:40-17:00 Coffee Break

17:00-17:25 Stability and activity of mesophilic subtilisin E and its thermophilic homolog: insights from molecular dynamics, Giorgio Colombo (Istituto di Biocatalisi e Riconoscimento Moleculare, Milano, Italy)

17:25-17:50 Novel compatible solutes from hyperthermophiles: application for stabilization of enzymes, Helena Santos (ITQB, Lisbon, Portugal)

17:50-18:15 Molecular design of novel metal-binding biomolecules, oligomeric metallothioneins, Yoshikatsu Murooka (Osaka University, Osaka, Japan)

19:30-23:00 Conference Dinner


WEDNESDAY, April 12, 2000


Session E: Bio-industrial and analytical applications: case studies

Chairman: Marie Dominique Legoy (NL) and Raquel Aires-Barros (P)

9:00-9:35 Stabilisation of a-amylases for starch and whole grain mash liquefaction, Sven Pedersen (Novo Nordisk, Bagsvaerd, Denmark)

9:35-10:10 Enzyme immobilisation on Eupergit C for redox biocatalysis, John Woodley (University College London, London, UK)

10:10-10:35 Stabilization of protein ligands, Martin Linhult (Royal Institute of Technology, Stockholm, Sweden)

10:35-10:55 Coffee Break

10:55-11:20 Stabilization of immobilized enzymes against organic solvents: Generation of "polymeric salts" surrounding enzyme molecules by combined use of cationic and anionic random coil polymers, José Guisan (CSIC, Madrid, Spain)

11:20-11:45 Application of solid/gas catalysis to the industrial production of esters sharing the natural label, Sylvain Lamare (University of La Rochelle, La Rochelle, France)

11:45-12:10 Transglutaminase catalyzed enzyme immobilization - an alternative approach for the development of biosensors for urea determination, Lutz Haalck (I. of Chemical and Biochemical Sensor Research, Münster, Germany)

12:10-12:30 Closing Session

Contact Addresses
General Information:

Dr Duarte Miguel Prazeres
Tel: +351 1 8419065
Fax: +351 1 841 9062

Web Page:

Supported by UNESCO / MIRCEN network
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