Title page
Financial support
Present address
Materials and Methods
Results and Discussion
Drawings and graphs
Nucleotide and aminoacid sequences


A technical note is an article giving a brief description of a technique or procedure. It may also refer to the modification of a technique, procedure or equipment of interest to Electronic Journal of Biotechnology.

It should be no more than 2500 words, and could include two figures or tables. It should have at least 8 references.

Technical notes are also send to peer review.

All manuscripts should be prepared with Microsoft Word and sent to the Editor by e-mail as "attachment" to the electronic address

A galley proof is provided to author(s) before the article is available for all audiences.

The articles are published in HTML and PDF formats.


It should contain the following information:

The full title of the paper without abbreviations. The title should be as brief and informative as possible, specifying clearly the content of the article.
If the title is long (more than 80 characters and spaces), a shortened running title having no more than 50 characters and spaces should be provided.
Full names (clearly indicate name and surname) of all authors indicating the corresponding authors.
Affiliation, telephone, fax, electronic addresses and URL's of personal and institutional WEB pages.


Authors must include the financial support received for research.


Authors must provide between three and six keywords, which must not be part of the title of the paper. Phrases and general or broad words such as "pH" or "growth" are not allowed.


Authors should provide their present address in case it is different than the affiliation described at the Title page. It should include address, phone and fax.


They should be indicated as in example:


AFLP: polymerase chain reaction
PCR: amplified fragment length polymorphism


The abstract of the manuscript should not exceed 350 words and must be structured into separate sections: Background, the context and purpose of the study; Results, the main findings; Conclusions, brief summary and potential implications. Please minimize the use of abbreviations and do not cite references in the abstract.

In case of review articles, the abstract should be submitted as one section.


It should be brief and limited to the definition of the problem, the aims and purposes of the research and its relation with other studies in the field. Also the working hypothesis must be clearly stated.


It should include relevant details on the experimental design and techniques so that the experiments can be repeated.


The aim of this section is the interpretation of the results and their relation to the existing knowledge. The contribution to Biotechnology must be clearly stated.


The acknowledgments of the contributions of colleagues can be stated in this section. Acknowledgments for financial support must be cited on the corresponding section.


For original articles (research, short communications, technical notes, biotechnology issues for developing countries), at least 75% of the references must be from the Science Citation Index Expanded and at the same time from the last decade. Also the DOI number must be included at the end of each reference. Citations from thesis, personal communications, and unpublished data are not allowed.

a) In the text:

References must be cited in the text mentioning the last name of the author and year between parenthesis. In case of two authors, both should be mentioned. When there are three or more authors, mention only the first author followed by et al. When two or more references are cited in the same parenthesis, the authors should be in chronological order. And if they have the same year, they should be in alphabetical order. Moreover, if there is more than one reference of the same author and the same year, they should be indicated with letters.

See examples:

  • 1 author: (Gardner, 1999).
  • 2 authors: (Larvol and Wilkerson, 1998).
  • 3 or more authors: (Frishman et al. 1998).
  • 2 or more references in the same parenthesis: (Benton, 1996; Zhang et al. 1997; Larvol and Wilkerson, 1998; Frishman et al. 2010).
  • 2 or more references with the same year: (Klevecz, 1999; Wedin, 1999; Persidis, 2000).
  • 2 or more references with the same author and the same year: (Benton, 1996a; Benton 1996b; Benton 1996c).
b) In the References section:

At the end of the paper, in the References section the literature should be arranged in alphabetical order. If they have the same author, they should be in chronological order. They must be presented according to the following examples:

Journal Article


GODOY-HERNÁNDEZ, G.; AVILÉS-BERZUNZA, E.; CARRILLO-PECH, M. and VÁSQUEZ-FLOTA, F. (2008). Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.). Electronic Journal of Biotechnology, vol. 11, no. 1. DOI: 10.2225/vol11-issue1-fulltext-3.

MIKSCH, G.; KLEIST, S.; FRIEHS, K. and FLASCHEL, E. (2002). Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors. Applied Microbiology and Biotechnology, vol. 59, no. 6, p. 685-694. DOI: 10.1007/s00253-002-1071-z.



AUSUBEL, F.M.; BRENT, R.; KINGSTON, R.E.; MOORE, D.D.; SEIDMAN, J.G.; SMITH, J.A. and STRUHL, K. (2002). Current Protocol in Molecular Biology. Wiley-Interscience. 410 p. ISBN 978-0-471-50338-5.

ZAR, J.H. Biostatistical Analysis. (1999). Englewood Cliffs, N.J., Prentice Hall Inc. 663 p. ISBN: 0-13-081542-X.

Book chapter:


IMHOFF, J.F. and TRIIPER, H.G. (1989). Purple nonsulfer bacteria. In: STALEY, J.T; BRYANT, M.P.; PFENNIG, N. and HOLT, J.G. eds. Bergey’s Manual of Systematic Bacteriology. Baltimore, Williams & Wilkins, vol. 3, p. 1658-1682.

PÉREZ, L.; LÓPEZ, S.; BELDARRAÍN, A.; ARENAL, D. and PENTON, E. (1994). Purification of recombinant Hepatitis B surface antigen (rec-rHBsAg) from P. Pastoris: A process development study. In: GALINDO, E. and RAMIREZ, O.R. eds. Advances in Bioprocess Engineering. Netherlands, Kluwer Academic Publishers, p. 241-246.

Paper in Conference Proceedings


ACEVEDO, F.; CACCIUTTOLO, M.A. and GENTINA, J.C. (1988).Comparative performance of stirred and Pachuca tanks in the bioleaching of a copper concentrate. In: Biohydrometallurgy: Proceedings of the International Biohydrometallurgy Symposium. (12th - 6th July, 1988, Warwick, UK). Science and Technology Letters, Kew Surrey, UK. NORRIS, P.R. and KELLY, D.P. eds., p. 385-394.

BOSTICCO, A. (2000). Scarcity and surplus of the animal production: possible strategies for a better worldly balance. In: International Congress: Food production and the quality of life. (4th - 8th September, 2000, Sassari, Italy). Abstracts, p. 16.

Journal Article In Press


DASILVA, E.J. (1998). Biotechnology: developing countries and globalization. World Journal of Microbiology and Biotechnology, vol. 14. In press.

HOLBROOK, C.C.; KVIEN, C.K. and RUCKER, K.S. (2000). Preharvest aflatoxin contamination in drought tolerant and drought intolerant peanut genotypes. Peanut Science. In press.


Tables must be numbered with Arabic numerals in the order in which they are cited in the text. They should have a brief descriptive title placed at the top. A short description is also accepted. Footnotes can be included below the table. Tables cannot duplicate data contained in the text. Tables must be sent in Microsoft Word and have no links to the main document or other archives. Provide files at approximately the correct size they are to be printed (letter size).




The figures (photographs, drawings) must be numbered with Arabic numerals. Footnotes can be included below the figure.

Figures that include more than one image should be labeled as a, b, c, etc. (lower case, use black or white bold according to the figure.). The figure must include brief descriptive title and a subtitle must be provided for each image.

Figure 5. Immunolocalization of SOD in root of pearl millet. Two-day-old resistant (IP 18293) pearl millet genotypes were inoculated with S. graminicola zoospores (4 x 104 zoospores/ml). Root portion of seedlings was excised 4 hrs of post inoculation and used for the sectioning. Primary antibody was used at a dilution of 1:5000. The reaction was observed as fluorescence under a wild Leitz fluorescence microscope attached with photoautomat.
(a) Light field.
(b) Autofluorescence of resistant uninoculated.
(c) Autofluorescence of resistant inoculated.



They should be cited as figures and must be numbered with Arabic numerals. Footnotes can be included below the figure.

Graphs that include more than one image should be labeled as a, b, c, etc. (Arial lower case).

They must be in 2D and have a title. In case they have legend, it must be placed below the image.

They should be built with Arial font and in the colour palette called “Trek” (warm earth colours). See page layout in MS Office 2007. For the Spanish version the colour palette is called “Viajes”.

Graphs must be send in editable formats (MS excel if possible) in order to make formal changes (colour, fonts, size, etc.).


Colum chart:

Line Chart:


Digitalized photographs must be sent using a TIFF format, with a minimum of 300 dpi. Also GIF and JPG formats can be used.

Special care on the maximum definition of the photographs is required.


For line art, the following software can be used: Excel, Power Point, Adobe Illustrator 8 EPS, Freehand 8, Canvas 6, and Postcript Level 2.


We accept digitized videos using mpg format.


New sequence data referred to in a manuscript should also be reported with their accession number assigned in the DDBJ/EMBL/GenBank sequence data libraries. A manuscript can be submitted while sequence data is being entered into the data libraries with the intent of including accession numbers once the manuscript is approved for publication.

Accession numbers are best presented in a separate paragraph at the end of the Materials and Methods section.

Addresses for the databases are: DDBJ; EMBL; GenBank.

Supported by UNESCO / MIRCEN network
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