Plant Biotechnology
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 13 No. 6, Issue of November 15, 2010
© 2010 by Pontificia Universidad Católica de Valparaíso -- Chile Received June 14, 2010 / Accepted August 28, 2010
DOI: 10.2225/vol13-issue6-fulltext-6  

Development of trinucleotide (GGC)n SSR markers in peanut (Arachis hypogaea L.) 

Mei Yuan1 · Limin Gong2 · Ronghua Meng3 · Shuangling Li1 · Phat Dang4 · Baozhu Guo5 · Guohao He*2 

1Shandong Peanut Research Institute, Qingdao, China
2Department of Agricultural Sciences, College of Agricultural, Environmental and Natural Sciences, Tuskegee University, Tuskegee, AL, USA
3Department of Pathology and Laboratory Medicine, School of Medicine, University of Pennsylvania, PA, USA
4USDA-ARS, National Peanut Research Laboratory, Dawson, GA, USA
5USDA-ARS, Crop Genetics and Breeding Research Unit, Tifton, GA, USA

*Corresponding author:

Financial support: This work was supported by a grant from the National High Technology Research and Development Program of China (No. 2006AA100106) and partly supported by USDA/CSREES/CBG (No. 00-38814-9541).

Keywords: cultivated peanut, microsatellites, polymorphism, simple sequence repeat.

Abstract   Full Text

Cultivated peanut (Arachis hypogaea L.) is an oilseed crop of economic importance. It is native to South America, and it is grown extensively in the semi-arid tropics of Asia, Africa, and Latin America. Given an extremely narrow genetic base, efforts are being made to develop simple sequence repeat (SSR) markers to provide useful genetic and genomic tools for the peanut research community. A SSR-enriched library to isolate trinucleotide (GGC)n SSRs in peanut was constructed. A total of 143 unique sequences containing (GGC)n repeats were identified. One hundred thirty eight primer pairs were successfully designed at the flanking regions of SSRs. A suitable polymerase was chosen to amplify these GC-rich sequences. Although a low level of polymorphism was observed in cultivated peanut by these new developed SSRs, a high level of transferability to wild species would be beneficial to increasing the number of SSRs in wild species.

Supported by UNESCO / MIRCEN network